Target enriched multiplex PCR (Tem–PCR), a novel technology applied in molecular biology, includes three reaction stages which are enriching, tagging and amplifying by super primers. The promising Tem–PCR method solves the incompatibility and preference during amplification in routine PCR and makes pathogeny synchronous detection in one reacting system come true, hence, it gives to a new clue for researches in clinical pathogenic differential diagnosis, genotyping and resistance genes. This paper summarized the situation of commercial kits, research background and reaction principle based on the review of the applications of Tem–PCR in domestic and abroad with the purpose to provide references for further research in the field and new ideas for dealing with public health crisis.