A eukaryotic expression vector of full-length gene SIRT1 was constructed and transfected into porcine primary ovary granulosa cells for investigating the effects of overexpression from gene SIRT1 on AMPK mRNA expression and its activity. The results showed that the full-length coding sequence of porcine gene SIRT1 was identical to NCBI reference sequence (EU030283.2), it had 2 229 bp. Both mRNA and protein expression level of gene SIRT1 were significantly increased in the group transfected with pEGFP–C1–SIRT1 (P<0.01). The mRNA expression of AMPK–α1 and AMPK–α2 were both significantly increased, so did the phosphorylation level at Thr172 sites in AMPKα (P<0.05). The overexpression of gene SIRT1 was significantly increased the AMPK expression and its activity in ovarian granulosa cells via in vitro cultured from primary porcine. These results suggested that SIRT1 might play an important role in regulating the apoptosis of porcine granulosa cell through activating AMPK.