To study the effect of the amino acid content on formation of nitrogen-containing compound in flue-cured tobacco during curing process, factor analysis and grey correlation analysis were used to investigate the relationship between amino acid content in tobacco leaf and contents of protein, total nitrogen, total alkaloids, NH3, HCN in cigarette gas during curing process. Results showed that the content of amino acid had a more substantial change in yellowing mid-stage to color-settings mid-term, and Pro, Ala, Arg and Met had a higher content. The 16 amino acids were divided into 4 categories according to the change of their content during curing process. Protein and total alkaloid fluctuated largely in content, contents of HCN and NH3 had a large variation in late yellowing stage(42?48 ℃), and the changes of the contents of total nitrogen and nitrate were relatively small. Gly had a high correlation with nitrogen-containing compounds (the correlation coefficient were 0.443 7, 0.442 6, 0.408 7, 0.398 1, 0.345 6, 0.364 7). Thr had the highest grey correlation with NH3 content in the cigarette smoking stream, which was 0.419 4. The relationships between Glu, Lys, Tyr and 6 nitrogen-containing compounds showed small differences. Val had a high correlation with the contents of total nitrogen, protein, nitrate and HCN in smoking stream, the correlation coefficients were 0.411 4, 0.456 4, 0.423 9, 0.402 3, respectively. Met had a high correlation with HCN content in smoking stream, with correlation coefficient 0.450 6.