In this study, cDNA encoded farnesyl diphosphate synthase (FPS) was cloned using homology RT–PCR method from Hedera helix leaves. The results revealed that sequence of the cDNA with 1 050 bp base had an open reading frame of 1 026 bp and 342 amino acid residues. The sequence of nucleotides and amino acids of FPS were homology with those of araliaceous plants; according to the online prediction, the FPS, with a 39 735.7 relative molecular mass of the protein, stored in cytoplasm and located in the outer membrane; the speculated FPS contained two conserved domains of aspartic acid, and its secondary structure was mainly dominated with alpha helix and random coil; phylogenetic analysis on amino acid sequence of the FPS in dicotyledons showed that the FPS was closely related to Eleutherococcus senticosus, Panax ginseng, Panax notoginseng, Panax quinquefolius and Aralia elata, which were consistent with the traditional classification rules.