PFP, a peroxidase family protein gene, was successfully separated and cloned from rice by means of RT–PCR strategy, overexpression construction was prepared and nine positive transgenic plants were obtained by gene gun bombardment method. Compared to Zhonghua17, the overexpression plants of the candidate gene PFP showed heat tolerance. Functional association analysis of the gene PFP using 33 rice cultivars showed that it had significant correlation (P≤0.01) with heat tolerance. The expression of gene PFP in salt, cold, PEG and ABA stress was also analysised in this study.