Fifty Huanglang chickens were selected to directly sequencing using the PCR products. Geographic distribution of the clades of chickens from China was analysis. The results showed that Huanglang had high levels of genetic diversity, with nucleotide diversity, nucleotide differences and haplotype diversity were 0.012 54±0.000 94, 6.569 and 0.886±0.035, respectively. In the 26 variable sites, 23 haplotypes were defined, 13 of which was new. These haplotypes were belonging to clade A, B, C and E, respectively, and E was dominated haplogroup. Phylogenetic analysis and geographic distribution of the clades suggested that Huanglang originated from north and southwest China, and the gene flow with neighbor chickens was contributed significantly to this breed formation.