For the purpose of exploring the impact of fish stocking impact on the diversity of arthropod community in the lotus field ecosystem, community component, density and diversity indices of arthropod in lotus field at Wugang city, Hunan province, China were investigated from April to September in 2014 under five different fish (crucian, carp, grass carp) stocking applications. The results showed that in the experimental lotus field in which a total of 61 species of arthropod belonging to 32 families and 9 orders were collected, there were 44 species of arthropod belonging to 21 families in the fish-stocking field when crucian, carp, grass carp were stocked at 2 400, 2 400, and 1 200 individuals/hm2, which was the most; the species number of arthropod was 39 in the field with no-fish stocking, which was the least compared to other applications.. The arthropod species increased with the increasing density of the stocking fish. Rhopalosiphum nymphaeae, Prodenia litura and Chironomidae were the major pests species under each applications, the amount of Rhopalosiphum nymphaeae and Chironomidae were markedly higher in fish-stocking fields than those in no-fishing stocking fields while the amount of Prodenia litura showed no significant difference among each applications, Pirata subpiraticus and other spiders acted as the primary natural enemies, and the amount of Pirata subpiraticus under no-fish stocking applications was significantly higher than those under fish stocking applications. Diversity and evenness indices in fish stocking applications were greatly higher compared to no-fishing stocking application, on the contrary advantage concentration was lower in fish stocking applications. Fish stocking applications showed the least similarity of arthropod community with no-fish application.