A full length of 933 bp cDNA sequence encoded with 310 amino acid residues of MlNAC2 was cloned from Miscanthus lutarioriparius. The theoretical molecular weight and isoelectric point of this protein were 34 427.8 and 5.85, respectively, and it could be classified to stable proteins with an instability index about 34.84. MlNAC2 was assumed as a hydrophilic protein for its protein sequence contained a conservative NAM motif and no signal peptide or transmembrane structure. The subcellular localization assay confirmed that MlNAC2 was located in the nucleus and therefore might function in this subcellular. Transactivation assay indicated that MlNAC2 was a transcription factor protein and the transactivation region located at its C–terminus. Result from real-time quantitative PCR test suggested that the transcript of MlNAC2 at the roots of Miscanthus lutarioriparius was up-regulated at high salt, drought, ABA, MeJA or mechanical injuries, on the contrary, it was down-regulated at cold, but did not give response to SA.