Two F2 generations, which from the hybridizations of C543×614 and C543×B137, were used as experiment materials to analyze the pollen fertility and inheritance. The results showed that C543 was pollen-free male sterility, which was controlled by a pair of recessive nuclear genes, and there was a restoring gene in restorer 614 and B137, respectively. With the analyzing of SSR, the restoring gene of B137 was mapped on short arm of chromosome 8 and 16.9 cM from the marker umc1483.