In this study, Heinuo 27 and Bainuo 66 from F2 generation of super black waxy corn inbred lines I46 crossed white waxy corn inbred lines BN13 were selected to study the distribution and accumulation regularity of maize pigment in grains by microscopic observation, solvent extraction method and spectrophotometry. The results showed that the pigment of super black waxy corn existed in seed coat, which appeared from the top of kernels and then spread to the whole grain. With the development of grains of Heinuo 27, the content of carotenoid decreased gradually, however, the content of anthocyanin increased until it reached the maximum value on the 30th day after pollination and then decreased. With the development of grains of Bainuo 66, there was no melanin in the grains and the contents of carotenoid increased first and then dropped, however, the anthocyanin was not detected in the whole grain development process.