Polyvinyl alcohol (PVA) film, a former prepared plastics from Galla chinensis was selected for studying its optimum preparation technics. 0.55 g，1.67 g and 5.00 g weight of Gallotannin extracts were proposed as low, medium and high dose group, respectively, UV spectrophotometry was employed to measure their content. The results showed that the mass concentration for each ingredient was proposed as follows: 0.55 g/(100 mL) of Galla chinensis for low dose group, 1.67 g/(100 mL) for medium dose group, and 5 g/(100 mL) for high dose group; the mass concentration of PVA1788 were 8 g/(100 mL) and the volume fraction of glycerol were 1% for each corresponding group, respectively; The volume fraction of Tween–80 were 4%, 5% and 4% for corresponding group, respectively; and the penetration enhancers were comprised of 10% of 1,2–propanediol, 8% of bomeol and 5% of azone for low dose group, 3% of azone, 5% of 1,2–propanediol and 8% of bomeol for medium dose group, and 10% of 1,2–propanediol, 8% of bomeolm and 5% of azone for high dose group. Linear relationship was observed between tannin concentration and absorbance through the analysis of UV spectrophotometry.