Using Lilium brownii var.viridulum Baker as female parent and Lilium davidii Duch.var.unicolor (Hong)Cotton and Lilium longiflorum Thunb as male parents respectively, the hybridizations were carried out through different pollination methods at different flowering period, then the immature ovules of 60 d, 86 d and 98 d after pollination were transfered to different mediums to rescue the embryoes of hybrids , later the hybrids were identified through RAPD. The results showed that 4 d after flowering was the optimum time for Lilium brownii var.viridulum Baker and Lilium davidii Duch.var.unicolor (Hong)Cotton to pollinate by cutting stigmas, while the traditional pollination method was helpful for hybridization of Lilium brownii var.viridulum Baker and Lilium longiflorum Thunb. The medium of 1/2MS+ 0.1 mg/LNAA+ 0.1 mg/L6–BA was the best for embryo culture and 98 d after pollination was the best time for culture . The F1 hybrids were identified via RAPD and indicated that new germplasm of lily (var.viridulum Baker and var.unicolor (Hong)Cotton, Thunb) could be gained by wide hybridization with appropriate method of pollination and embryo rescue.