ISSR analysis was conducted on 5 redried cigarettes which were made from 5 tobacco cultivars (Honghuadajinyuan, Yun87, NC297, NC102 and K326). The result showed that these redried cigarettes were differentiated by 5 ISSR primers (826, 836, 841, 847 and 856), based on which the fingerprints of the 5 redried cigarettes were constructed using primers of 826 and 856, respectively. With primer 826, PCR bands in six levels from 250 bp to 1 000 bp were obtained, and with primer 856, PCR bands in five levels from 750 bp to 2 000 bp were obtained.