Two temperature treatments (4 ℃, room temperature for CK) and four plant growth substances of GA3, IAA, 6-BA and 2,4-D ( each of them had four mass concentrations of 50, 100, 200, 100 mg/L, take water as CK) were designed to study the effects of low temperature and plant growth substance on the seed germination of Miscanthus lutarioriparius.The results were as the follow: Compared with CK, 4 ℃ treatment could significantly improve the seed germination rate, germination potential and extremely significantly improve the germination index, vigor index and root length, but did not significantly improve bud length; All mass concentration of GA3 could significantly improve the germination rate, germination potential, germination index, vigor index and bud length, which the concentration of 100 mg/L had the most effect on germination; except 400 mg/L concentration, the other concentrations of IAA could improve the germination rate, germination potential, vigor index and promote root growth, the concentration of 50 mg/L was the best among them; The mass concentration of 6–BA could significantly improve the germination rate, germination index, germination potential and bud growth, but significantly reduce seed vigor index and the root growth; the effects of 2,4-D on germination rate, germination potential and germination index were rise first then falling down with the increase of the concentration, all mass concentration of 2,4–D could extremely significantly reduce vigor index and the root growth, 200 mg/L concentration was the best among them.