The whole open reading frame (ORF) sequence of a membrane-associated NAC transcription factor gene, HbNTL2, was cloned and analyzed by using reverse transcription polymerase chain reaction (RT–PCR) and Real-time PCR, and yeast was adopted as a material in the transactivation assay. The results indicated that HbNTL2 belonged to the ANAC001 subfamily with an ORF of 1 380 bp and encoded a protein with 459 amino acids. A conserved NAC domain (12–167) and a transmembrane motif (433–453) were respectively identified at the N–terminal and C–terminal from motif analysis, the result of which indicated that HbNTL2 was a membrane-associated protein. Results from transactivation assay in yeast cells demonstrated that HbNTL2 assumed the function for transcription activator, and its activation domain was located at the highly divergent C–terminus. The expression level of HbNTL2 in leaves was higher than that in other parts of the rubber tree，and could be induced by tapping, jasmonic acid (JA), ethyphon (ET) and cold stress. The results suggested that HbNTL2 might be play important roles in the regulation of growth, development and stress responses.