Blueberry seedlings (Blomidon) were chosen and three irrigation treaments ( relative soil water content of 30%–45%, ＞45%–60% and ＞60% as TR1, TR2 and TR3, respectively) and a control (non irrigation as CK) were designed to investigate the changes of soil nutrient, organic matter content agronomic traits, chlorophyll fluorescent characteristics of leaves and fruit quality of blueberry. The results showed that TR2 could significantly increase the content of available phosphorus and organic matter in blueberry rhizosphere soil, while had no significant effect on soil alkali hydrolysable N and available potassium, however, when relative soil water content exceeded 60%, alkali hydrolysable N and organic matter in soil decreased. Irrigation decreased the content of anthocyanin, vitaminc in blueberry fruit by varying degrees and had on effect of fruit acidity, but increased the total sugar contentand ratio of sugarto acid, TR2 had the highest content of total sugar in fruit. ＞45%–60% of relative soil water content was the optimal irrigation for blueberry (Blomidon), considering the effects of different irrigation treatments on the growth parameters, photosynthetic parameters and fruit quality.