To study the inhibition effect of red raspberry on photo-damage of HaCaT cells induced by ultraviolet radiation B (UVB), HaCaT cells were first incubated for six hours with three different doses of red raspberry juice (20, 40，80 mg/mL), and then irradiated with 60 mJ/cm2 dose of UVB. Cell viability was detected by MTT method. The level of malondialdehyde (MDA), hydroxyproline ( Hyp ) and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH–Px), lactate dehydrogenase (LDH) in supernatants were determined with colorimetric method. The content of intra-cellular reactive oxygen species (ROS) was tested with fluorescence method. Compared with the control group, the results showed that the UVB irradiation could seriously damage HaCaT cells, which lead to 29.54% of cell viability decline and obviously decrease the activities of SOD and GSH–Px (P<0.01). Compared with the UVB group, red raspberry of the three doses could obviously enhance cell viability, respectively (P<0.05 or P<0.01), and could increase activities of SOD and GSH–Px, as well as Hyp content, but it could decrease activity of LDH and MDA, and ROS contents in supernatants with UVB irradiation at dose-dependent manner (P<0.01 or P<0.05), which could decrease the rate of cell damage and apoptosis. In conclusion, red raspberry could relieve oxidative damage and apoptosis of HaCaT cells by UVB–induced and exhibit a photo-protection effect, which might be the reason that red raspberry could efficiently enhance the oxidase activity, clear the oxy-radicals and stimulate the collagen synthesis in cells.