The combining ability of resistance to sugarcane brown stripe disease was analyzed based on 23 female parents (ROC 5 for control) and 21 male parents (69–463 for control) )and their 29 crosse combinations(ROC 5×69–463 for control) ). The results indicated that difference of the combining ability among the female parents, male parents and crosse combinations was significant, which was mainly controlled by additive and non–additive gene. The average heritability of resistance to sugarcane brown stripe disease was female parents（95.3%）,cross combinations（93.0%）and male parents (79.1%). The general combining ability of 12 female parents, 10 male parentsshowed negative effect, which female parents were Pma 98–40, Yacheng 93–26, Yunrui 05–283, Yuetang 91–976, Chuangzhe 19, ROC 10, Yunzhe 06–80, ROC 26, Zhanzhe 74–141, K 86–110,Yunzhe 03–194 and ROC 25. male parents were Q 199, Yunrui 06–649, Yunrui 05–733, CP 84–1198, CP 88–1762,Yacheng 84–125,Yunrui 05–784,Yuetang 00–236, CP 72–3591 and CP 94–110. The special combining ability of 16 crosses combinations showed negative effect, they were Pma98–40×Yunrui05–649, Yacheng93–26× Yunrui 05–733, Yunrui 05–283×Q199,Yuetang 91–976×CP 84–1198,Chuanzhe 19×CP 88–1762 and ROC 10×Yuenong 73– 204.