A light-inducible gene was cloned and named by StR2R3-MYB1, which had a full length of 798 bp ORF, encoded a R2R3–MYB transcriptional factor with 265 amino acids, and located in number 10 chromosome of potato. It was showed from the amino acid sequence analysis that StR2R3–MYB1 contained two DNA–binding domains which were composed of two tandem myb motifs. There were a large number of light associated components such as G–Box, Box4, Box II, I–Box, MNF1, ABRE (components induced by abscisic acid), Circadian (components related to biological clock) and CGTCA (components related to methyl jasmonate). It was not founded the transmembrane signal and the coiled coil in amino acid sequence of StR2R3–MYB1, while, it had an obvious hydrophilic region and protein signal on N terminal. R2R3–MYB–YFP, the fusion protein was localized at cell nucleus. StR2R3–MYB1 was homologous with proteins R2R3–MYB in tomato (SlCMYB1) and eggplant (SmMYB) with the similarity to 99% and more than 70% homology with Arabidopsis thaliana (AtMYB113). Tissue specific analysis indicated that StR2R3–MYB1 expressed in root, stem and leaf. Semi-quantitative and real-time RT–PCR revealed that the StR2R3–MYB1expression was induced by white, far red and blue light.