The regional differences of neutral aroma components of flue-cured tobacco leaves were studied on C3F tobacco samples collected from 26 sampling points in Henan from 2011 to 2013. The results showed that the contents of carotenoid degradation products in tobacco leaves from Luoyang except linalool and megastigmatrienone3 were all significantly lower compared to other regions; the contents of Millard reaction components of Luoyang except 2–acetyl pyrrole and 5–methyl furfural were also significantly lower. The contents of PHE degradation products in tobacco leaves from Xuchang except benzyl alcohol were significantly higher than those from other regions. The solanone contents in tobacco leaves from Nanyang and Sanmenxia were both significantly lower than those from Zhumadian while higher than those of Luohe. The neophytadiene contents in tobacco leaves showed no significant difference among different sampling points. The total scores of neutral aroma components in tobacco leaves from the South were high, from the North were low while those from the East were high, from the West were low.