To screen manganous-salt resistant bacteria for the microbial treatment of manganese polluted environment. The soil samples were collected from the manganese ore yard and manganese tailing dams in the Xiangtan Manganese Industry Co. Lid.. The manganous salt-resistant bacteria were isolated on manganous sulfate containing solid plate. The morphology characteristics and phylogenic analysis of the 16S rDNA sequence were used for the identification of the strain KM01. The flame atomic absorption method for the determination of the manganese concentration in centrifuged broths containing 0.005 mol/L, 0.01 mol/L and 0.015 mol/L of manganous sulfate was used to calculate the removal rate of manganese. Through an orthogonal experimental design, the liquid inocula of KM01 were directly inoculated into the crude pig-breeding fecal wastewater was used to detect the manganese removal capacity of the strain. 1) A manganous salt-resistant bacterium KM01 was isolated and identified as Serratia sp. 2) In the broths containing manganous sulfate, KM01 could biosorb manganous ion and produce dark brown precipitate. The removal rate of manganese reaches 94.8%, 95.8% and 89.1%, respectively. 3) The removal rate of manganese in the wastewater reaches 80.5% under the condition of 28 ℃, pH 7 and 1% of inoculum size. The manganous salt-resistant strain Serratia sp. KM01 has potential application in the precipitation and removal of manganese in organic wastewater containing manganous ion the same as pig-breeding fecal wastewater.