生猪伪狂犬病毒gB抗体与中和抗体的相关性分析
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长沙市科技成果产业化重点项目(K1308135–31)


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    摘要:

    为探索生猪伪狂犬病毒gB–ELISA抗体水平的高低与中和抗体效价的相关性,运用酶联免疫吸附测定(ELISA)法对177份生猪血清进行了伪狂犬病毒gB抗体的检测,同时,用中和试验对上述血清样品进行了伪狂犬病毒中和抗体的测定。试验结果通过生物统计学分析,gE抗体阴性血清的gB抗体平均S/P值和中和抗体效价(SN效价)倒数平均值的相关系数为0.926,说明gB免疫抗体S/P值与其中和抗体效价有很好的相关性。中和抗体水平的高低一般与免疫保护力呈正相关,研究结果表明gB–ELISA抗体水平可以用来评估生猪对当前流行PRV毒株的相对免疫保护力。

    Abstract:

    The PRV gB-antibody value from 177 serum samples were detected using the approach of ELISA and the PRV neutralizing antibody titer in the same samples were measured through neutralization test to discuss their correlationship. The result from biostatistics showed that the correlation coefficient between mean S/P value of gB-antibody and neutralizing antibody titer in negative serum of gE-antibody was 0.926, which indicated that S/P value of gB-antibody and neutralizing antibody titer in immune serums have a good correlation. Generally speaking, neutralizing antibody titer had a positive correlation with protective immunity, which showed that S/P value of gB-antibody could act as an index to evaluate the porcine protective immunity for the current epidemic PRV.

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.生猪伪狂犬病毒gB抗体与中和抗体的相关性分析[J].湖南农业大学学报:自然科学版,2015,41(3):.

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  • 在线发布日期: 2015-05-29
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