Taking the MS basal medium without any hormones addition as control, the germination rate, shoot induction rate, number of inducted shoots and bulblets were measured by using the seeds of Lilium brownii F. E. Brown ex Miellez var. giganteum as sterile sowing materials, which were sowed in different concentration of hormones mediums and treated with peeling.The results showed that seeds adopted here had higher viability, as well as those without peeling. Treatment with peeling could speed up seed germination and germination rate. The optimum culture medium for seed germination was MS medium supplemented with 1.0 mg/L 6–BA and 1.0 mg/L NAA.The germination rate, induction rate and the number of average indefinite buds were 100.0%, 65.0% and 1.28, respectively. Besides, the number of average indefinite bulblets, leaves and roots after 45 days of transferred them to MS basal medium were 2.35, 3.6 and 6.7, respectively.