The effects of different seedling ways(normal floating system, shallow water floating system, seedling nursing on moist trays, plate airing and seedbed fermentation floating system) on the growth and physiological and biochemical indexes of burley tobacco were observed. The results showed thatcompared to the normal floating way, the water temperature was raised by 4.5 ℃ with seedling nursing on moist trays, the media temperature was raised by 1.99 ℃ with plate airing, and the seedbed period wasshortened by 7 days with 3 cm shallow water and 6 dayswith plate airing. In root period, compared to other seedling ways, the number of leaves and max leaf length and width of tobacco seedlings nursing on moist trays were better, the number of lateral roots and secondary lateral roots of tobacco seedlings with plate airing were significantly superior. In seedling period, there was no significant difference onnumber of leaves and secondary root, above- and below-ground dry weight, chlorophyll content, and root activity and SOD activity of tobacco seedlings among different seedling ways; theaboveground fresh weight of tobacco seedlings with seedbed fermentation was superior to those with other seedling ways; the number of secondary lateral roots, underground fresh weight, nitrate reductase activity and seedling rate of tobacco seedlings were superior to those with other seedling ways. The cost of seedling using shallow water floating and normal floating were the lowest, whichwas 696 Yuan/hm2, and seedlingcost using seedbed fermentation was the highest, which increased by 576 Yuan/hm2compared to normal floating.