To decrease the exploitation of the non-renewable resources as peat, vermiculite, expanded perlite used in traditional medium, and reduce the seedling cost of tobacco, tobacco float seedling test is done with medium containing mixture of tillage layer soil, burnt soil, farmyard manure, and nutrient fertilizer. The result showed that the tobacco seedling medium with 34% tillage layer soil, 34% burnt soil, 30% farmyard manure, and 2%–2.5% nutrient fertilizer, was close to the traditional medium concerning physicochemical indexes and could produce robust tobacco seedlings. The tobacco seedling emergence rate reached 84.57%, and the qualified seedling percentage reached 97.53% with this new medium. There are small difference between the new and the traditional medium on growth and agronomic trait. And the seeding cost was saved 48 yuan per pool compared with the traditional medium.