Effects of physical restriction to ineffective tiller on non-structural-carbohydrates accumulation (NSC) and yields were studied by a pot experiment with water culture based on two rice cultivars, Peizataifeng and Yuejingsimiao. Physical restriction were conducted by designing different transplanting holes, they were 20 mm, 28 mm and 35 mm, denoted as D1, D2 and D3, respectively. Compared to D1 and D3, percentage of valid panicle, total grain number per panicle and yields of Peizataifeng and Yuejingsimiao from D1 were significantly increased. NSC in stem and sheath from D2 and D3were remarkably higher than those of D1. The larger leaf area and NSC in stem and sheath of Peizataifeng and Yuejingsimiao were from D3. However, the higher NSC in stem and sheath of Peizataifeng and Yuejingsimiao were mainly transferred to vegetative organ, instead of to yield formation. And there was also significantly positive correlation between total grain number per panicle and NSC in stem and sheath at panicle initiation stage of Peizataifeng and Yuejingsimiao.