In order to evaluate land eco-security of nine cities in Wanjiang district, a comprehensive assessment method on the normal cloud model is suggested. Firstly, assessment index system is constructed, and land eco-security grade is described through single index for each districts. Secondly, each index weight is calculated by entropy weight method, and each eco-security grade evaluated by corresponding indexes is described using three mathematical characteristics of the normal cloud model. Thirdly, according to each assessment index, matrix of membership cloud model for each grade is determined using positive cloud generator, then, the fuzzy transformation subset is obtained based on the weights and membership degree matrix. Finally, the biggest membership degree is chosen as the result of comprehensive evaluation, and all results are compared with that of by entropy weight and matter element model. The results show that the land eco-security of Chuzhou, Hefei, Xuancheng are in safe grade, Wuhu, Tongling, Chaohu in relative safe grade, Anqing in critical safe grade, Chizhou in less safe grade, and Ma’anshan in high safe grade. Therefore, the land eco-security grade of nine cities in Wanjiang district belongs to the average level on the whole, which is most consistent with the result assessed by matter element model.