Sample-plot survey method was conducted to study the biodiversity of ant species from the tea gardens in main tea-growing areas of Chongqing. A total of 30 species of Formicidae including 9 dominating species were identified in the tea gardens in main tea-growing areas of Chongqing. The species richness of ant communities from the tea gardens at different altitudes were 3-12 species, Shannon-Wiener diversity indexes were 0.15-1.64, Pielou evenness indexes were 0.09-0.74, and Simpsom dominant indexes were 0.09-0.77.