Taking cultivar K326 as the sample, effects of different production management modes (conventional mode, organic mode) on neutral and acidic aroma content of middle and upper flue-cured tobacco leaves were studied. The result showed that organic mode had a significant or very significant higher content of neutral and acidic aroma components in upper middle leaves after cured compared to conventional mode. β–ionone and dihydroactinidiolide content with organic mode was significant higher than those with conventional mode in the middle leaf while isophorone oxide content was very significant lower than those with conventional mode. β–damascenone and other 8 kinds of neutral aroma components with organic mode was significant higher than those with conventional mode in the upper leaf while megastigmatrienone B and other 5 kinds of neutral aroma components content was very significant higher than those with conventional mode. Decanoate content with organic mode was significant higher than that with conventional mode while monoprop acidic aroma components content was very significant higher than that with conventional mode in middle leaf. In upper leaf, phenylacetic acid, 3–methyl valerate and 4–methyl valerate content with organic mode was significant higher than those with conventional mode. Content of carotenoid degradation products, cembratriendiol atabolites and neophytadiene in upper leaf with organic mode was significant higher than those with conventional mode, but there was no difference in middle leaf. Therefore, the organic mode was more beneficial for improving aroma composition content of upper leaf. Main effects analysis showed that, leaf position was the main factor, production management model was the sub-factor, but both position and production management model had a significant effect on content of neutral aroma composition.