Resistance of 18 new maize combinations to stalk rot caused by Fusarium graminearum was investigated by artificial inoculation of soil in the field. The results showed that the occurrence of the stalk rot disease was various in different combinations after inoculated with the pathogen. Two combinations were resistant to stalk rot, accounting for 11.1% of the total combinations; 4 combinations showed moderate resistance, accounting for 22.2%; 2 combinations were susceptible, accounting for 11.1%; and 10 combinations were highly susceptible, accounting for 55.6%. Among these combinations, the incidence for combination B36 and B38 was 8.3%, which reached resistance level against stalk rot. Simultaneously, the yield of 18 combinations under inoculated and non-inoculated conditions was analyzed, the yield of B36 and B38 under non-inoculated were 8 691.0 and 8 362.5 kg/hm2, respectively; and 6 760.5 and 7 012.5 kg/hm2 respectively under inoculated, which indicated combination B36 and B38 was resistant to stalk rot with stable and high yield.