A simple vector construction procedure, allowing rapidly and efficiently clone DNA fragments into cloning vector, was recommended in this study by taken target DNA fragments, Ha–FP–A and Ha–FP–B from Heliocoverpa armigera single nucleocapsid nucleopolyhedro virus (HaSNPV) of G4, green fluorescent protein gene (GFP) and an appropriate cloning vector of G4FPAGB1 as examples. Fragment of them were digested as follows: Ha–FP–A by Hin d Ⅲ and Eco RⅤ, Ha–FP–B by XhoⅠand Bam HⅠ, GFP by Eco RⅤand XhoⅠ, and the vector by Hin d Ⅲ and Bam HⅠ, respectively. All these fragments and vectors were determined with electrophoresis first, and then extracted from the gel at the same tube. Following the ligation system developed, the mixture of target DNA fragments was automatically ligated and transformed. The Results showed that the 3 target DNA fragments were all successfully ligated to the plasmid vector. Therefore, this method was simple, efficient and universal for the recombination of plasmid vector production.