Based on GPS technique, 362 tobacco soil samples were collected in Luoping, methods of geo-statistics and classical statistics were used to investigate the spatial variability and supplying availability of soil main nutrients in tobacco-growing field. The results indicate the nugget to sill ratio of soil organic and main nutrients in Luoping tobacco-growing field is 25%-75%, which is in the range of moderate spatial dependence. Spatial variability of those main nutrients was caused by structural and random factors. As the spatial distribution maps show, average content of soil organic matter is 42.23%, and the content of organic matter in 52.27% of the tobacco-growing soil was over 40 g/kg; average content of total N and available N were 2.02 g/kg and 161.96 mg/kg respectively, content of total N of 51.39% tobacco-growing soil was over 2 g/kg and available N content in 79.84% of tobacco-growing soil was above 150 mg/kg; average content of total P and available P were 1.63 g/kg and 38.45 mg/kg respectively, total P content in 11% of the tobacco-growing soil was above 2 g/kg and available P content in 29.79% of the tobacco-growing soil was above 40 mg/kg; content of total K was below 12 g/kg; average content of available K was 257.23 mg/kg, and available K content in 95.87% of the tobacco-growing soil was above 150 mg/kg.