Descriptive statistical analysis methods were used to investigate the regional layout change of flue-cured tobacco planting from 1983 to 2012 in China from two aspects, one aspect was North and South tobacco growing region, the other was Southwest tobacco growing region including Southeast tobacco growing region, the upper reaches of the Yangtze River region, Huanghuai tobacco growing region, North tobacco growing region. The results indicated that from 1983 to 2012, the migration tendency of domestic tobacco planting layout was moved gradually from North to South, especially to southwest. On this basis, variance selection, theory hypotheses and model construction were conducted according to the agricultural location theory and producer behavior theory and eviews was used analyze the impact factors of flue-cured tobacco planting layout change from 1983 to 2012 in China. The results indicated that the planting layout change of tobacco in flue-cured tobacco main planting regions of China had correlation with land resource, geography and climate, economic condition, technological progress and national policies. For Huanghuai main planting region, the most obvious factors were per capita arable land, grain supply of household, technological progress, national policies and employment opportunity besides farming for famers. For Southwest main planting region, the most obvious factors were grain supply of household, technological progress, national policies and natural disasters.