稻米蛋白组分含量的品种差异及其与米质的关系
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湖南省研究生科研创新项目(CX2012B278);国家教育部“创新团队发展计划”项目(IRT1239);湖南省教育厅项目(KC2011B023)


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    摘要:

    在相同栽培条件下种植45个水稻品种(系),分析其谷蛋白、醇溶蛋白、清蛋白、球蛋白等4种蛋白质含量和总蛋白含量,及其与糙米率、精米率、整精米率、精米长与长宽比、垩白粒率、垩白度、碱消值、胶稠度、直链淀粉含量等米质性状指标的关系,获得了如下主要研究结果:1)不同水稻品种(系)的4种蛋白质含量中,谷蛋白的含量最高,其绝对含量平均值为7.84%,相对含量平均值为78.04%,约占总蛋白含量的80%,醇溶蛋白、清蛋白和球蛋白的含量分别占总蛋白含量的10.58%、8.17%和2.76%。2)不同水稻品种(系)的4种蛋白质含量存在明显的品种(系)间差异,蛋白质含量的变异系数从大到小依次为球蛋白、醇溶蛋白、清蛋白、谷蛋白。3)总蛋白含量与谷蛋白含量、醇溶蛋白含量、清蛋白含量均呈极显著正相关,其相关系数分别为0.93、0.60和0.55,总蛋白含量与球蛋白含量呈显著正相关,其相关系数为0.32;不同水稻品种(系)的谷蛋白含量除了与醇溶蛋白含量呈显著正相关(r=0.34)外,与其余各蛋白质含量的相关关系均无统计学意义;醇溶蛋白含量与清蛋白含量呈极显著正相关(r=0.71),与球蛋白含量呈显著正相关(r=029);清蛋白含量与球蛋白含量呈极显著正相关(r=0.44)。4)不同水稻品种(系)的谷蛋白、醇溶蛋白含量对米质性状的影响比较大,而清蛋白、球蛋白含量对各米质性状的影响不大;不同水稻品种谷蛋白含量与垩白粒率、垩白度均呈极显著负相关(相关系数分别为–0.50和–0.47),稻米醇溶蛋白含量与精米率、整精米率均呈极显著正相关(相关系数分别为0.40和0.58)。

    Abstract:

    In the present study, 45 rice cultivars (lines) were chosen to test four protein components of glutelin, globulin, prolamin, albumin and 11 rice qualities including brown rice rate, milled rice ratio, head rice ratio, grain length, grain length/width, chalky rice rate, chalkiness, alkali spreading value, gel consistency, amylase content and protein content. The main results were gained as follows: 1) Glutenin content was the highest among the 4 protein components in different rice cultivars (lines), its average absolute and relative content was 7.84% and 78.04%, respectively, with a proportion of 80% in the total protein content. The prolamin content was higher than that of others with about 10.58% of the total protein content. The albumin and globulin content was the lowest, according for 8.17% and 2.76% of the total protein content, respectively. 2)There were significant difference among the 4 kinds of protein components of different rice cultivars (lines), the coefficient of variation was ranked by globulin, prolamin, albumin, glutelin in turn. 3)There were positive significant or very significant correlation between the total protein content and the protein content of 4 components in different rice cultivars (lines). The correlation coefficient between the total protein content and glutenin, prolamin, albumin, globulin content were 0.93, 0.60, 0.55, 0.32 respectively, which attained the very significant level except for globulin with the significant level. The positive correlation between glutenin and prolamin content was respectively reached the significant level (r=0.34), while the correlation between prolamin content and albumin, globulin content were reached the very significant level (r=0.71) and the significant level (r=029). The correlation between albumin and globulin content was reached positive very significant correlation (r=0.44). 4) The glutelin and prolamin content had close relation with rice quality in different rice cultivars (lines), albumin and globulin content, while, were demonstrated little influence on rice quality. The correlation coefficient (r = –0.50, –0.47 ) between glutelin and chalky rice rate, chalkiness were all reached negative very significant level, while, correlation coefficient (r =0.40, 0.58) between prolamin and milled rice ratio, head rice ratio were all reached positive very significant level.

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徐庆国,童浩,胡晋豪,刘红梅,李先喆.稻米蛋白组分含量的品种差异及其与米质的关系[J].湖南农业大学学报:自然科学版,2015,41(1):.

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  • 在线发布日期: 2015-01-28
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