In order to provide the standard for quality control of Ginkgo biloba leaves scientifically, metabonomics was used to observe the change rules of chemical components in Ginkgo leaves in different seasons. Extract from Ginkgo biloba leaves harvested in different seasons (spring, summer and fall) was obtained by the standard extraction technology. The data was acquired by MSE method using UPLC-QTOF, which was processed to compare the component differences between different groups of Ginkgo biloba leaves by means of principal component analysis. The result shows that component differences of Ginkgo biloba leaves in different harvest seasons are obvious, and they changed regularly with time,and the content of quercetin 3-O-2’’-(6’’’-p-coumaroyl) glucosyl rhamnoside showed a clear downtrend from spring to summer and fall.