Eighteen samples of flue-cured tobacco K326 upper leaves (B2F), middle leaves (C3F) and lower leaves(X2F) of Hubei xiangyang were collected and the content of Pb, Cd,Cr, Ni and As and their distribution characteristics were determined. The results showed that the average content of Cd in tobacco leaves was the highest, followed by Pb, Ni, Cr and As. The contents of Pb, Cd, Cr and As in old leaves and in leaf area with fewer dry mass were high, in main vein were low, Ni content in the main vein was higher than that in other leaf part. The distribution of Pb, Cd, Ni was not as uniform as that of Cr and As. Contents of Pb, Cr and As were extremely significantly positive–correlated. The contents of Ni and other elements showed a negative correlation.