We collected 214 sera samples from a farm which its growing-finishing pigs with poor health conditions and detected porcine parvovirus (PPV) by PCR. A sample contained PPV identified by PCR was inoculated into ST cells to isolate PPV and the isolate was sequenced and we described partial of its biological characteristics. We found that there were no sera contain PPV detected by PCR from pigs aged from 2 to 14 weeks and 24 week, however, PPV can be detected from serum of pigs aged from 15 to 18 and 22 weeks with positive rate 10%, 40%, 20%, 15.4% and 9%, respectively. The isolate was serially passaged in ST cells and the ST showed stable cytopathic effects when the isolate passaged to the fifth generation. Hemagglutination titer of the isolate was 28 by the hemagglutination assay. We also found that the partial genome of the isolate share more than 98% identity with PPV genomes found in GenBank, especially as high as 99.1% with the strain YL which was isolated by Shi[1] in 2012. Therefore, we demonstrated that the isolate is PPV and nursing pigs in late stage as well as fattening pigs in early stage have a high PPV rate in this farm.