The root inherited characters of sugarcane seedlings were conducted by a potted trial. The results indicated that root length, root crossings were significantly affected by male, female and their combination; root surface areas were significantly affected by male and female, and root dry weights were significantly affected by their combination. The contribution sequence of heritability on offspring decreased as female, their combination and male in turn. The heritability of root crossings, root length, root surface areas, number of root tips, root dry weight and root volume were all greater than 60%. The root characters were much better for these springs by taken ROC22 and GT92–66 with female and CP72–1210 and CT 89–103 with male. The correlation analysis showed that the root length, root surface areas and root diameter might be used as the index for selecting roots in sugarcane breeding.