Arsenic contents in different parts (upper, middle and lower leaves) and micro-regions (leaf base, leaf middle, leaf tip, leaf margin and leaf primary vein) of tobacco leaf of the flue-cured tobacco, burley tobacco and Maryland tobacco from Hubei province were determined. The results showed that the arsenic content in lower leaves were the highest, while in upper leaves the lowest; in micro-regions, leaf tip, leaf base and leaf margin showed higher arsenic content, followed by leaf middle, and leaf primary vein showed the lowest arsenic content; arsenic content in micro- regions of the lower leaves was high, while in micro-regions of the upper leaves was low.