According to Arcanobacterium pyogenes plo gene sequences published on GenBank, six specific loop-mediated isothermal amplification (LAMP) primers were designed to establish a method of LAMP for detection of Arcanobacterium pyogenes from forest musk deer and the specificity and sensitivity of this method were assayed. The specific test results showed that positive results obtained only from Arcanobacterium pyogenes while the amplified results of 8 non-Arcanobacterium pyogenes strains including Salmonella typhimurium, non-O1 Vibrio cholerae, Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus, Aeromonas hydrophila, Vibrio parahemolyticus and Bacillus cereus were negative. The detection sensitivity of the LAMP assay for Arcanobacterium pyogenes was 112 fg pure genomic DNA per 25 μL reaction. The test based on the method of this essay were applied on 20 clinical samples, 10 clinical samples were positive which were in accord with API coryne identification.