Fifty weaned healthy piglets with similar weight were randomly assigned to five groups: control group fed with corn-soybean meal diets during all the process; test group 1, 2, 4 and 4 first fed with basal diet added with probiotics containing Bacillus cereus, Lactobacillus rhamnosus, and Lactobacillus pentosus mixed in ratio of 1∶1∶1, 1∶2∶1, 2∶1∶2 and 1∶1∶2, respectively from the 1st to the 28th day of the experiment, then with basal diet from the 29th day to the 42nd day. Daily gain, feed/weight ratio, diarrhea rate were calculated after 28 days of feeding. The results showed that daily gain was higher, feed/weight ratio was lower and diarrhea rate was significantly lower in piglets of test groups compared to the control. Daily gain of piglets in test group 1 and 3 was significantly higher than that in the control and in test group 1 was slightly higher than in group 3. Feed/weight ratio of piglets in test group 1 and 3 was significantly lower than that in the control and in test group 1 was slightly lower than in group 3. Piglets in test group 1 and 3 showed the same diarrhea rate which was lower compared to test group 2 and 4. These results indicate probiotics with mixing ratio of 1∶1∶1 and 2∶1∶2 have better growth promoting effect for piglets. Quantitative PCR was applied on samples of test group 1 and control group collected after 0, 7, 14, 21, 28, 42 d from the beginning of the experiment, the results showed Bacillus cereus, Lactobacillus rhamnosus, and Lactobacillus pentosus in intestine of piglets in test group 1 exhibited the same growth tendency during feeding the probiotics, which increased and then decreased and increased again, while the 3 strains in intestine of piglets of control group didn’t show such same tendency. The 3 strains still showed regular changes 2 weeks after ceased feeding the probiotics, indicating probiotics could regulate the growth of the correspond bacteria in intestine of the piglets. Denaturing gradient gel electrophoresis (DGGE) and sequencing of 16SrDNA showed that Bacillus cereus, Enterococcus, Streptococcus, Bifidobacterium, Lactobacillus pentosus, Escherichia coli and Lactobacillus rhamnosus decreased 7 days after feeding probiotics, and increased 28 days after feeding probiotics, showing probiotics could not only regulate the growth of the correspond strain, but also the growth of Enterococcus, Streptococcus, Escherichia coli and Bifidobacterium in the intestine.