细辛根系浸提液和腐解液对小麦和黄瓜的化感效应
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国家“十二?五”科技支撑计划项目(2010BAI03B01)


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    采用室内生物测定法及基质盆栽试验,研究不同质量浓度的细辛根系浸提液(Asarum heterotroidees root extracts,ARE)和根系腐解液(Asarum heterotroidees root decomposition,ARD)以及灭菌根系腐解液(sterilized ARD, SARD)对小麦和黄瓜种子萌发及幼苗生长的影响,并测定其根系活力、叶片中蛋白质和叶绿素的含量等生理指标,探索细辛对2种受体作物的化感效应。结果表明:①25 mg/mL(低浓度)细辛根系浸提液对2种受体植物的种子萌发均无明显影响,100 mg/mL(高浓度)处理对受体的种子萌发和幼苗生长均有明显抑制作用;不同质量浓度的细辛根系浸提液均降低了黄瓜和小麦的根系活力、蛋白质及叶绿素含量,且随着质量浓度的增大,其抑制作用增强。②细辛根系腐解液处理对受体植物种子萌发、幼苗生长及生理活动的抑制程度均显著高于同质量浓度的根系浸提液处理(P<0.05),高、中(50 mg/mL)低浓度根系腐解液处理后黄瓜的根系活力分别比对照降低了25.5%,43.8%和62.8%。③同质量浓度的灭菌根系腐解液处理对二者生长的影响明显低于未灭菌腐解液处理,3种处理液的化感抑制效应表现为根系腐解液处理组最强,根系浸提液处理组最弱。细辛可以通过残根腐解的形式向土壤中释放某些化感物质来干扰小麦和黄瓜早期的生长及生理过程,微生物可能影响细辛根系腐解物的化感活性。

    Abstract:

    Effects of various contents of Asarum heterotroidees root extracts (ARE) and Asarum heterotroidees root decomposition (ARD) and sterilized ARD (SARD) on seed germination and seedling growth of wheat and cucumber were conducted using approach of biological assays and pot experiments. Allelopathic effects of ARE and ARD on the two receptor plants were also carried out by adopted roots activities, germination rate, seedling growth, content of protein & chlorophyll and other related physiological indexes as indicators. The result showed that there were no significant effects of ARE on seed germinations of wheat and cucumber at lower concentrations (25 mg/mL), significant effects, while, were occurred at higher concentration (100 mg/mL). Root activities, content of protein & chlorophyll declined with the concentration of ARE increase. The inhibition effects of ARD on seed germination, seedling growth and some physiological activities of the two plants were significantly higher than those treated by ARE (P<0.05). The activity of cucumber roots with respective high, moderate (50 mg/mL) and low concentrations treatments were declined by 25.5%, 43.8% and 62.8% compared to that in ARD, respectively. The inhibition effects could be ranked by ARD, SARD, ARE in order with three treatments. In general, some allelochemicals decomposed from A. heterotroidees roots might be released into soil, which would interfere in the early growth and physiological metabolism of wheat and cucumber. However, microbes might affect the allelopathic activity of decomposed products from A.heterotroidees roots.

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张秋菊.细辛根系浸提液和腐解液对小麦和黄瓜的化感效应[J].湖南农业大学学报:自然科学版,2014,40(3):.

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  • 最后修改日期:2014-06-01
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  • 在线发布日期: 2014-06-07
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