Yunyan97 was used as material to define the effect of different harvest maturities on appearance quality, chemical composition, smoking quality and economic characters of flue-cured tobacco with strong aromatic planted in rice paddy in South Hunan under the condition of bulk curing. The results showed that with the increasing of maturity degree, reducing sugar content and fresh/fry ratio of tobacco leaves increased gradually, the starch content decreasing gradually, the content of nicotine and total nitrogen of upper and middle leaves decreased slightly, and appearance quality, sensory quality and average price of cured tobacco leaves increased at first and then decreased. Upper leaves were suitable to be harvested when leaf surface was all yellow (90% to 100% yellow) and main veins all became white and yellow spots appeared on the leaf surface. Middle leaves were suitable to be harvested when leaves surface was pale yellow (80% to 90% yellow) and 1/2 of midrib length became white. Lower leaves were suitable to be harvested when leaf surface was yellow-green (60% to 70% yellow) and 1/3 of the midrib length became white. Chemical composition of these leaves was coordinate with high percentage of fine tobacco and high average price, appearance quality and smoking quality.