Two fungi strains with resistance to copper were isolated from red soil polluted by copper based on dilution plate method. The results from both morphology observation and ITS sequence analysis showed that the two fungi strains were Paecilomyces lilacinus and Aspergillus terreus. They both could endure copper concentration 300 mg/L at most. By added 10 g/L fungi strains into pH 5, temperature 25 ℃ culture medium with 10 mg/L Cu2+ and oscillated for 30 min with 120 r/min frequency, the removal rate of Cu2+ by P. lilacinus and A. terreus were 85.45% and 87.54%, respectively.