We have analysed the content and coordination of the main chemical substances such as nicotine, total sugar of the tobaccos with different oil states from the main cultivated tobacco variety K326 in eight counties of Guizhou province. The result shows as follows: For the carbohydrates and nitrogenous compounds, the contents of nicotine, total nitrogen and protein of the high oil tobacco are lower than those of low oil tobacco, but the contents of total sugar and reducing sugar of the high oil tobacco are higher than those of low oil tobacco, and their Shmuck value is higher, their sugar alkali proportion is more coordinated. It also shows that chloride ion content of the high oil tobacco is low, their potassium chloride content is relatively high. In conclusion, the high oil tobacco is of higher-quality material basis than the low oil tobacco.