The purpose of this study was to analyse the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene among Chabertia ovina isolates from goat in Shannxi province, China and to study its phylogenetic relationships with other nematodes using the pcox1 gene sequences. The partial cox1 (pcox1) were amplified from individual C. ovina sample by PCR. The amplified pcox1 sequences were aligned using the ClustalX 1.81. Then MP tree was constructed using the software PAUP 4.0. The lengths of all the pcox1 sequences were 393bp. Phylogenetic analyses showed that all the C. ovina samples from Shannxi province were clustered in the same clade. Sequences significant variation in pcox1within C. ovina is limited (0.5%-2.0%), while inter-species variation is obvious (9.4%-18.8%). Thus, the pcox1 sequences can be used as a genetic marker for population genetic studies of nematodes. The present study firstly reported the mitochondrial cox1 sequence of C. ovina. And the results of this study provided foundation for population genetic studies of C. ovina.