For better extracting and utilizing phospholipids in eggs of large yellow croaker (Pseudosciaena crocea), enzymolysis method was adopted here to improve their yield. On the base of single factor experiment, the optimal parameters for phospholipids extraction were determined by response surface method. Meanwhile, the extraction effects were compared with solvent extraction and ultrasonic method. The results showed that the optimal parameters were as follows: endonuclease papain with a ratio of solid to liquid 1∶2, pH 6.0, hydrolysis temperature 45 ℃, hydrolysis time 1.5 h, enzyme dosage 125 U/g. The average extraction yield of the phospholipids could be reached up to 70.92%. The results also indicated that the obtained parameters using response surface methodology was feasible for practical estimation. Compared with solvent extraction and ultrasonic extraction methods, the yield of phospholipids using enzymolysis method was improved 22.02%, 9.6% respectively in scale-up experiment. These results suggested that endonuclease papain aided extraction technology was one of the effective ways to improve the yield of phospholipids from the eggs of large yellow croaker.