Four land use types (secondary forest land (CL), shrub land (GC), pasture land (MC) and corn field (YM)) were selected to research their effects on soil organic carbon and nutrients in karst region at Northwest of Guangxi province. The results showed that the content of soil organic matter (SOM) and total N (TN) in shrub land and secondary forest land were higher 38.5%100% and 63%130% than those of in pasture land and corn field, respectively. The contents of soil total P (TP) and total K (TK) in different land use types were significant difference. Soil available P (AP) content was mainly affected by fertilization, while soil available K (AK) content was controlled by vegetation cover and water & soil loss. Land use types were the dominant factors affecting the content of SOM, TN, TP and TK. Extensive cultivation could decrease soil nutrients content and resulted in soil degradation of cropland, on the contrast, ecological restoration could improve soil fertility. Therefore, in karst region, the measures as changing extensive cultivation into intensive farming, applying organic fertilizers, balance fertilization, and vegetation restoration will keep the sustainable utilization of land resources.