MlNAC13 gene was cloned from Miscanthus lutarioriparius (M. lutarioriparius), and subcellular localization and transactivation assay were conducted on this gene. Then real-time quantitative PCR (RT–qPCR) was used to analyze the expression profile of MlNAC13 in root under salt, drought, cold, abscisic acid (ABA), Methyl jasmonate (MeJA) and Salicylic acid (SA) treatments. The results showed that 1) complete MlNAC13 gene sequence of M. lutaroriparius was obtained; 2) subcellular localization analysis using N-terminus fused with YFP showed that MlNAC13 was located in nucleus, suggesting that it might function in nucleus; 3) transactivation assay indicated that the full-length and C-terminus of MlNAC13 had transactivation activity, indicating that MlNAC13 is a transcription factor and the transactivation activity region located in C-terminus; 4) the transcript of MlNAC13 was up-regulated under all surveyed treatments except SA, indicating that it might participate in various stress responses of M. lutarioriparius.