The function of AtCS82 gene in Arabidopsis is still unknown, but it is supposed to be associated with seed oil anabolism. To investigate the function of AtCS82 gene, semi-quantitative RT-PCR was used to analyze the expression of AtCS82 gene in different tissues of Arabidopsis (stem, leaf, flower, pod), the results show expression of AtCS82 gene was high in pod, but low in stem, leaf and flower, indicating the participation of AtCS82 gene in seed development. Three primers were used to screen homozygous mutants with T-DNA insertion, and the expression of AtCS82 gene in homozygous mutant plant was tested using RT-PCR, the results proved the obtaining of stable pure mutant with AtCS82 gene silenced. AtCS82-overexpressing transgenic plants were obtained by constructing pCAMBIA1300–35S::CS82 recombinant plasmid, followed by transforming into Arabidopsis thaliana of Columbia ecotype (Col).